It Takes 2: RNA–DNA Mashup May Have Kick-Started Life on Earth

From Scientific American:

According to the RNA world hypothesis, early life used RNA to carry genetic information and perform biochemical catalytic reactions. Over time, DNA developed from RNA as the carrier of genetic information and proteins appeared as biochemical catalysts.

As RNA gave way to DNA, some think a mixture of nucleotide building blocks would have been inevitable. As these nucleotides connected to form strands, the thermodynamic and kinetic stability of pure RNA and DNA duplexes would drive these nucleic acids to accumulate in primitive cells, while less thermally stable complexes containing one strand of RNA and one strand of DNA fell apart.

Ramanarayanan Krishnamurthy, at the Scripps Research Institute, and colleagues wondered if duplexes where each strand contains both RNA and DNA nucleotides were stable enough to have been possible intermediates during the transition from RNA to DNA. The researchers purchased commercially synthesised sequences of RNA and DNA, six to 16 bases long. In some sequences, they systematically changed purine RNA nucleotides, containing the bases adenine or guanine, into the corresponding DNA purines. In others, they changed pyrimidine RNA nucleotides, with cytidine or uracil bases, into pyrimidine DNA nucleotides with cytidine or thymidine bases. The result was several series of nucleic acid duplexes that ranged from having all RNA nucleotides to none at all.

To test the thermal stability of these sequences, the researchers heated each duplex to separate the strands. Then they measured the increase in UV absorption as the strands melted. The faster the absorption increased as the temperature increased, the faster the strands separated, indicating a less stable duplex. Many mixed duplexes with strands containing both RNA and DNA nucleotides melted as much as 20 degrees before pure RNA or pure DNA duplexes, indicating they were significantly less thermally stable than the pure duplexes.

Krishnamurthy says they were surprised to see …

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